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04-A:Histology Question Paper

04-A:Histology

Course:

Institution: Kenya Medical Training College question papers

Exam Year:2008

KENYA MEDICAL TRAINING COLLEGE

DEPARTMENT OF MEDICAL LABORATORY SCIENCES

COLLEGE EXAMINATION 2009

BASIC DIPLOMA IN MEDICAL LABORATORY SCIENCES

PAPER:04-A HISTOLOGY (100 MARKS)

INSTRUCTIONS
1. Write you index number on every answer sheet used.
2. Do not write your name on any answer sheet.
3. Attempt both sections I and II

SECTION I COMPRISES OF M.C.Q.
4. Circle the correct answer(s) in every question.
5. Be careful,as half a mark will be deducted for every wrong response.

SECTION II COMPRISES OF ESSAY QUESTIONS
6. Attempt all the questions.Make sure you understand the question before answering.

SECTION I (M.C.Q 40 MARKS)
1.The following are the roles of the endoplasmic reticulum:
a)Transport
b)Synthesis
c)Protection
d)Cell division
e)Production of ATP
2.Plasma membrane is made up of two primary building blocks namely:
a)Vitamin
b)Carbohydrates
c)Proteins
d)Yolk
e)Lipids
3.Simple cuboidal epithelium are normally locatedin
a)Tympanic membrane
b)Salivary gland
c)Ovary
d)Blood vessels
e)Thyroid vesicles
4.Prickle cells are found in the:
a)Bladder
b)Skin
c)Adrenal glands
d)Lungs
e)Pancreas
5.Which one of the following fixatives is/are associated with electron microscope:
a)Formadehyde
b)Gluteraldehyde
c)Picric acid
d)Osmium tetra oxide
e)Carnoy
6.Potasium Dichromate is exceptionally good for demonstration of:
a)Phosphatase
b)Chromaffin
c)Lipase
d)RBC
e)Enzymes
7.Micro anatomical fixatives include?
a)Bouins
b)Acetone
c)Carnoys
d)Clarke''s
e)Zenker
8.Surface de-calcification is done on a tissue:
a)After detection of traces of calcium that can destroy microtome knife
b)Before de-calcification is done
c)that is exceptionally hard
d)that is examined using electron microscope
e)that was not fixed
9.Why are chelating agents not used routinely as a means of de-calcification?
a)They are very expensive
b)They are very slow
c)They are easily burnt out
d)Zeocarb 255 is not readily available
e)They cause conjuctivitis
10.Which one of the following mineral acids does not require to be rinsed using 1% urea
a)Perenyl fluid
b)Nitric acid
c)Nitric acid formaldehyde
d)Formic acid
e)Trichloacetic acid
11.One of the following mineral acids causes tissues to float:
a)Toluene
b)Chloroform
c)Cedar wood oil
d)Benzene
e)Aniline oil
12.The boiling point of soft paraffin wax is:
a)45-50
b)55-60
c)54-56
d)37-40
e)46-48
13.What are the advantages of using automatic tissue processing:
a)It can process the tissue even when it is not the normal working hours
b)Time to process the tissue is considerably reduced
c)Automatic tissue processing machine is cheap to purchase
d)It is only operated by experts
e)Can be operated even when there is no electricity
14.Filter system used in fluorescence microscope includes
a)Barrier
b)Polarizer
c)Analyzer
d)Nicol prism
e)Exciter
15.Which factors affect dark ground microscope negatively during tissue examination
a)Use of standard objective
b)Many specimens
c)Dust
d)Inadequate source of light
e)Clean slide
16.The refractive index of microscope slide is:
a)0.5
b)1.51
c)15.1
d)0.151
e)151
17.Which one of the following types of knives is/are used in electron microscope
a)Glass
b)Diamond
c)Plain wedge
d)Ben Heiffor
e)Plano concave
18.Base Sledge Microtome is good for
a)Lung
b)Brain
c)Bones
d)Eyes
e)Teeth
19.The following types of hones are the finest
a)Arkansas
b)Carborudum
c)Strop per
d)Belgium Yellow
e)Belgium Black
20.Which one of the following is/are possible causes of section detachment from the slide?
a)Blood clot
b)Pieces of bones
c)Acids
d)Preservatives
e)Very soft tissues
21.Orcein dyes are extracted from
a)Cochineal bug
b)Saffron flowers
c)Lecanora Tinctoria
d)Litmus plant
e)Rocella Tinctoria
22.Metachromatic dyes include:
a)Thionin
b)Haematoxylin
c)Urantia
d)Neutral red
e)Carbol Fuschin
23.The role of glycerine in the Ehrlich haematoxylin staining techniques is:
a)It is a solvent
b)It is a dye
c)It is a mordant
d)It is a stabilizer
e)It is a preservative
24.Pink pigment can be removed by:
a)Running tap water
b)20% Nitric acid
c)2% Nitric acid
d)70% Alcohol
e)0.2% Nitric acid
25.Melanin can best be demonstrated by:
a)DOPA
b)PPB
c)H&E
d)Von Kossa
e)Masson Trichrome
26.Kaiser Ling solution H in mainly used for
a)Fixation
b)Staining
c)To adjust ph
d)Colour restoration
e)To hydrolyse the tissue

SECTION II (ESSAY)
1.Explain in detail on the main types of staining processes (20 marks)
2.a)Tabulate the differences between chatters and scores (10 marks)
b)Discuss chelating agents as a means of de-calcification (10 marks)
3.Discuss the following
a)Possible causes of section detachment from the slide (5 marks)
b)Preparation of DPX (5 marks)
c)Main features of automatic tissue processor (5 marks)
d)Post chroming (5 marks)

04-A:Histology Question Paper

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