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04-A:Histology Question Paper

04-A:Histology

Course:

Institution: Kenya Medical Training College question papers

Exam Year:2008

KENYA MEDICAL TRAINING COLLEGE

DEPARTMENT OF MEDICAL LABORATORY SCIENCES

COLLEGE EXAMINATION 2008

BASIC DIPLOMA IN MEDICAL LABORATORY SCIENCES

PAPER:04-A HISTOLOGY (100 MARKS)

INSTRUCTIONS
1. Write you index number on every answer sheet used.
2. Do not write your name on any answer sheet.
3. Attempt both sections I and II

SECTION I COMPRISES OF M.C.Q.
4. Circle the correct answer(s) in every question.
5. Be careful,as half a mark will be deducted for every wrong response.

SECTION II COMPRISES OF ESSAY QUESTIONS
6. Attempt all the questions.Make sure you understand the question before answering.

SECTION I (M.C.Q 40 MARKS)
1.The following are cytoplasmic inclusions except:
a)Mucin
b)Vacuoles
c)Golgi apparatus
d)Yolk
e)Centrioles
2.Simple columnar epithelial cells are found in the:
a)Alimentary canal
b)Lungs
c)Gall bladder
d)Brain
e)Retina of the eye
3.The following features are found in the spleen:
a)Red pulp
b)White pulp
c)Kupffer cells
d)Goblet cells
e)Paneth cells
4.Characteristics of glycogen undergoing autolysis are:
a)Diminishes
b)Karyolysis
c)Pyknosis
d)Empty spaces
e)Become granular
5.Which one of the following fixatives is criteria of a good fixative:
a)Must fortify the tissue against harsh effect of solution to be used later
b)Must render tissue substances soluble
c)Must activate enzymes
d)Must have negative effect on staining
e)Must penetrate evenly and quickly
6.The following is associated with Osmic acid:
a)Preserve cytoplasm
b)Used in dark ground microscopy
c)Causes conjuctivitis
d)Storage in fume cup board
e)Penetrate quickly and evenly
7.The following is/are tissue softener
a)Formaldehyde
b)Penenyl fluid
c)Orths fluid
d)Mullers fluid
e)Clarkes
8.Nucleus is present in all types of cells except
a)White blood cells
b)Platelets
c)Red blood cells
d)Lymphocytes
e)Basophils
9.Post chroming helps to demonstrate:
a)Mitochondria and myolin
b)RNA and DNA
c)Lysosomes and golgi apparatus
d)Centrioles and mitochondria
e)Mucin and DNA
10.Polysterine resin used ion exchange include
a)Zeokarb 250
b)Formic acid
c)Zeokarb 225
d)Nitric acid
e)Urea
11.The following are characteristics of a good de-calcifying solution except:
a)Should damage the tissue
b)Reasonable speed of de-calcification
c)Should completely remove calcium
d)Impairment of subsequent staining technique
e)Should not damage the tissue
12.Dioxane:
a)Causes shrinkage
b)Is miscible with water
c)Used routinely
d)Is immiscible with water
e)Has got some characteristics as ethanol
13.Synthetic resins are mainly used for?
a)Soft specimen
b)De-calcified bones
c)Unde-calcified bones
d)For sections to be examined using electron microscope
e)For sections with different consistency
14.The following are found in polarized microscope:
a)Exciter filter
b)Barrier filter
c)Polarizer
d)Analyser
e)Ur light
15.The difference between phase contrast microscopy and interference microscope is:
a)Phase contrast microscope exaggerates minute difference in refractive index whereas interference does not.
b)Phase contrast utilizes short wave length against interference microscope
c)Phase contrast uses dark illumination unlike interference
d)Phase contrast produces varieties of brightness unlike interferences
e)Interference generates its own light rays whereas phase contrast does not
16.Vacuum impregnation is exceptionally good for the following tissue:
a)Heart
b)Fetus
c)Lung
d)Breast
e)Intestine
17.Which one of the following will cause the tissue to float on the surface?
a)Toulene
b)Chloroform
c)Xylene
d)Benzene
e)Carbon tetrachloride
18.Cryostat microtome is good for:
a)Frozen section
b)Brain
c)Liver
d)Fatty tissues
e)Hard tissues
19.The following are used in plate glass honing:
a)Hanging
b)Flexible
c)Medium abrasive powder
d)Fine abrasive powder
e)Carborudum
20.Starch paste is not used in the demonstration of:
a)Carbohydrates
b)Lipids
c)Proteins
d)Vitamins
e)Nucleus
21.Litmus dye is obtained from:
a)Saffron
b)Lichen
c)Coccus cacti
d)Crocus sativa
e)Rocella Tinctoria
22.Polysterine in DPX is:
a)Distrene
b)Butylpthalate
c)Xylene
d)Styrene
e)Xam
23.The following are agentaffin cells:
a)Brain
b)Eye
c)Melanin
d)Adrenal gland
e)Phenolic compound found in Kultschitzyky cells
24.Accentuators include:
a)Phenol
b)Picric acid
c)Carbol fuschin
d)Orange G
e)Carmic acid
25.Examples of mordants are:
a)Toluidine blue
b)Copper
c)Thiazine
d)Iron
e)Thionine

SECTION II (ESSAY)
1.Describe diagrammatically how a flourescence microscope functions (20 marks)
2.a)Describe physical theory of staining (10 marks)
b)Briefly explain how inorganic iron haemosiderin in ferric state is demonstrated (10 marks)
3.Explain the following terminologies
a)Corpora amylacea(5 marks)
b)Accentuators (5 marks)
c)Elective solubility(5 marks)
d)Causes of section detachment from slide(5 marks)

04-A:Histology Question Paper

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