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04-A:Histology Question Paper

04-A:Histology

Course:

Institution: Kenya Medical Training College question papers

Exam Year:2008

KENYA MEDICAL TRAINING COLLEGE

DEPARTMENT OF MEDICAL LABORATORY SCIENCES

2007 SEPTEMBER CLASS

MLS CLASS END OF TERM II EXAMS

BASIC DIPLOMA IN MEDICAL LABORATORY SCIENCES

HISTOLOGY PAPER

22/7/2008

8.30AM-10.30AM

PAPER:04-A HISTOLOGY (100 MARKS)

INSTRUCTIONS TO THE CANDIDATE
1. Write you index number on every answer sheet used.
2. Do not write your name on any answer sheet.
3. Attempt both sections I and II

SECTION I COMPRISES OF M.C.Q.
4. Circle the correct answer(s) in every question.
5. Be careful,as half a mark will be deducted for every wrong response.

SECTION II COMPRISES OF ESSAY QUESTIONS
6. Attempt all the questions.Make sure you understand the question before answering.

SECTION I (M.C.Q 40 MARKS)
1.Plasma membrane is made up of the following:
a)Alot of carbohydrates
b)Proteins
c)Vitamins
d)Lipids
e)Starch
2.The first phase of mitosis is:
a)Telophase
b)Metaphase
c)Meiosis
d)Anaphase
e)Prophase
3.The following types of cells are found in small intestines :
a)Kupffer cells
b)Goblet cells
c)Prickle cells
d)Paneth cells
e)Beta cells
4.Supra renal gland is situated:
a)Below the kidney
b)At the base of urinary bladder
c)In the kidney
d)Around the kidney
e)Slightly above the kidney
5.Pituitary gland is made up of the following except:
a)Zymogen granules
b)Chromophils
c)Sebum
d)Chromophobes
e)Pars nervosa
6.Osmium tetra-oxide is good for preservation of:
a)Lipids
b)Proteins
c)Chromaffins
d)DNA
e)Mitochondria
7.Schaudins fixative is made up of the following:
a)Saturated aqueous mercuric chloride
b)Distilled water
c)Absolute ethanol
d)Formalin
e)Picric acid
8.The following are found in both Hellys fluid and zenker except:
a)Mercuric chloride
b)Potassium dichromate
c)Sodium sulphate
d)Formaldehyde
e)Ethanoic acid
9.Yellow colouration in the tissue on de-calcification is as a result of:
a)Urea
b)Nitric acid
c)Chromic acid
d)Formic acid
e)Hydrochloric acid
10.Means of decalcification include:
a)Probing with pins
b)Felling with hands
c)Mineral acids
d)Chelating agents
e)X-ray
11.Factors that increase the rate of impregnation include :
a)The type of tissue
b)Agitation
c)Medium used
d)PH
e)Heat
12.The melting point of ester wax is:
a)46-48
b)50-55
c)55-60
d)37-40
e)52-54
13.All of the following are clearing agents except:
a)Butanol
b)Xylene
c)Toulene
d)Benzene
e)Dioxane
14.Two types of filters used in fluorescence microscopy include:
a)Microscope objective
b)Condenser
c)Exciter
d)Barrier
e)UV-lamp
15.Nicole prism is used in:
a)Phase contrast microscopy
b)Interference microscopy
c)Polarized microscopy
d)Electron microscopy
e)Dark ground microscopy
16.Tool wedge knife is exceptionally good for:
a)Unde-calcified bones
b)Soft tissues
c)Brain
d)Lungs
e)Liver
17.Section crumbling is as a result of:
a)Paraffin wax being too soft
b)Tilting knife too much
c)The rate of sectioning being too slow
d)Microtome vibration
e)Knife too blunt
18.The purpose of adhesive application is:
a)To stop tissue from cracking
b)To ensure sections are firmly attached to the slide
c)For permanent preservation
d)To increase refractive index
e)For easy storage
19.Aqueous mounting media include:
a)Farrant media
b)Xylene
c)Xam
d)Turpentine
e)Eurparol
20.Pancreas cells and granules can be demonstrated in:
a)Haematoxylin and eosin demonstration technique
b)Aldehyde fuschin technique
c)Papanicolou staining technique
d)Pearl acid schiff technique
e)Somori staining technique
21.In phosphotungstic acid haematoxylin the ripening agents include:
a)Sun
b)Mercuric chloride
c)Mercuric oxide
d)Sodium
e)Potassium sulphate
22.Mercury pigments are as a result of:
a)Zenker fixative
b)Helly fixative
c)Acetone
d)Trichloacetic acid
e)Bouins fluid
23.Harris can best be used:
a)As a nuclear stain
b)As a cytoplasmic stain in foots technique
c)As a cytology stain in papanicolou staining
d)As a progressive stain
e)Multinucleation
24.Criteria of abnormal cells include:
a)Single nucleus
b)Normal cytoplasm
c)Increase in nuclear cytoplasm ratio
d)Reduced nuclear cytoplasm ratio
e)Multinucleation
25.The following are for colour restoration in museum technique:
a)Kaiser Ling I
b)Kaiser Ling II
c)Spirit
d)Water
e)Kaiser Ling IV

SECTION II (ESSAY 60 marks)
1.a)List four phases of mitosis (4 marks)
b)What are qualities of good fixatives?(6 marks)
c)Write down the procedure of end point of de-calcification (10 marks)
2.With the aid of a well labelled diagram discuss vacuum impregnation technique(20 marks)
3.Define the following terms (10 marks)
a)stropping
b)serial section
c)accentuators
d)post chroming
e)putrefaction
4.Write short notes on rotary microtome (10 marks)

04-A:Histology Question Paper

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