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04-A:Histology Question Paper

04-A:Histology

Course:

Institution: Kenya Medical Training College question papers

Exam Year:2010

KENYA MEDICAL TRAINING COLLEGE KITUI
DEPARTMENT OF MEDICAL LABORATORY SCIENCES
P.O BOX 711
KITUI

SEPTEMBER 2008 MLS CLASS

HISTOLOGY PAPER
15/1/2010

TIME-2HOURS

INSTRUCTIONS TO THE CANDIDATE
1.Write your college number on every answer sheet used.
2.Do not write your name on any answer sheet.
3.Attempt both section I and II (section I comprises M.C.Q 40 points)
4.Circle the correct answer(s) in every question.
5.Be careful as half a mark will be deducted for every wrong response.
6.Section II comprises of essay questions (60 points). Attempt all the
questions and make sure you understand the question before you
understand it.

SECTION I:(MCQ 40 MARKS)
1.The role of centrioles includes:
a)excretion
b)cell division
c)transport
d)energy synthesis
e)production of enzymes
2.The first phase of mitotic division is:
a)prophase
b)anaphase
c)metaphase
d)telophase
e)meiosis
3.Cytoplasmic inclusions include:
a)golgi apparatus
b)lysosome
c)mucin
d)vacuoles
e)ground substance
4.Transitional epithelium are mainly found in?
a)ureter
b)bladder
c)bronchiole
d)small intestine
e)gall bladder
5.The kidney is mainly made up of two parts namely:-
a)serous coat
b)mucuos coat
c)muscular coat
d)cortex
e)medulla
6.The two types of cells that make up chromophil of pituitary gland
include:
a)eosinophil
b)pars nervosa
c)alpha cells
d)beta cells
e)delta cells
7.Acetone is a special fixative used mainly for the demonstration of:
a)chromosomes
b)nucleus
c)lysosomes
d)phosphate
e)lipase
8.Picric acid is a peculiar fixative in that:
a)it causes conjuctities
b)it precipitates proteins
c)it is used as a fixative,stain and in differentiation
d)it is explosive and has to be stored under water
e)it is radio opaque
9.Post-chromitization is:
a)treatment of tissue with 3% potassium dichromate after normal fixation
b)unmasking fatty substances
c)treatment of tissue with potassium dichromate in order to improve
mitochondria staining reaction
d)washing tissue sections in water
e)is done before fixation
10.The following are cytoplamic fixatives except:
a)clarkes fluid
b)mullers fluid
c)orths fluid
d)schandins fluids
e)carnoys fluid
11.Chelating agents include:
a)EDTA
b)Varsene
c)Zeokarb
d)Trichroacetic acid
e)Formal saline
12.X-ray method or testing end point of de-calcification is not applicable in tissues fixed in
a)formalin
b)zenker fluid
c)picric acid
d)mercuric acid
e)chromic acid
13.Clearing agents include:
a)butanol
b)xylene
c)toluene
d)ethanol
e)acetone
14.Which of the following processing techniques is best for lung tissue?
a)Gelatin embedding technique
b)Vacuum embedding technique
c)freezing embedding technique
d)L.V.N
e)Celloidin embedding technique
15.Soft paraffin wax has a boiling point of:
a)40-45
b)54-56
c)55-60
d)45-50
e)60-70
16.Specific gravity of chloroform is:
a)01.489
b)0.866
c)0.927
d)168
e)61
17.The following is/are jerk microtome:
a)Cambridge microtome
b)Rotary microtome
c)Freezing microtome
d)Rocking microtome
e)Cryostat microtome
18.Ultra microtome is good for tissue to be examined through:
a)light microscope
b)dark ground microscope
c)flurence microscope
d)polarized microscope
e)electron microscope
19.The common type of rotary microscope is:
a)tool edge
b)biconcave
c)plane wedge knife
d)piano concave knife
e)Ben heiffor knife
20.Stroppers are mainly mounted on:
a)flexible base
b)hanging base
c)hone
d)jeweler ronge
e)stone
21.The remedies of section crumbling include?
a)reduce the knife tilt
b)section the tissues more rapidly
c)apply ice to block the cool
d)clean the knife edge
e)hone the knife
22.Why is starch knife not routinely used to demonstrate carbohydrates?
a)gives false positive results
b)due to hot water added to the paste
c)the preservative always reacts with the carbohydrate
d)due to egg albumin which has carbohydrate potential
e)there is no gelatin
23.Orcein dyes are obtained from:
a)lecarona tinctoria
b)rocella tinctoria
c)saffron flower
d)hard wood
e)coccuscati
24.Main chromophores used in synthetic dyes include:
a)alcohol
b)haematin
c)draining
d)haematoxylin
e)orange G
25.Aqueous medium include:
a)glycerine
b)xylene
c)xam
d)DPX
e)DPS

SECTION II (60 marks)
1.Explain in details the main types of staining processes (20 marks)
2.a)Discuss chemical test of end point of de-calcification (10 marks)
b)Discuss rotary microtome(10 marks)
3.Write short notes on: (20marks)
a)Ion exchange.
b)Post chromatization.
c)Features of automatic tissue processors.
d)Physical theory of staining.

04-A:Histology Question Paper

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