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Food And Water Microbiology Question Paper

Food And Water Microbiology

Course:Bachelor Of Science

Institution: Kenyatta University question papers

Exam Year:2009

KENYATTA UNIVERSITY
UNIVERSITY EXAMINATIONS 2009/2010
FIRST SEMESTER EXAMINATION FOR THE DEGREE OF BACHELOR OF
SCIENCE
HML 404:
FOOD AND WATER MICROBIOLOGY

DATE: Monday, 28th December, 2009

TIME: 2.00 p.m. – 4.00 p.m.
------------------------------------------------------------------------------------------------------------
INSTRUCTIONS:
SECTION A
Answer ALL questions (10 marks each)
1.
a)
State the family name of the following water borne viruses
i)
Rotavirus
ii)
Human corona virus
iii)
Hepatitis A virus
iv)
Poliovirus
(4 marks)

b)
Explain the sources of carbon and light for the following organisms
i)
Chemolithotrophs
(chemoautotroph)
(2
marks)
ii)
Photoheterotrophs
(2
marks)

2.
a)
Explain
the
term
Coliform.
(2
marks)

b)
State four reasons for choosing Escherichia coli as an ideal coliform
during
water
analysis.
(4
marks)

c)
Outline the indole test for Escherichia coli.
(4
marks)

3.
a)
Outline milk pasteurization using Ultra High Temperature technique.

(3 marks)
Page 1 of 2

b)
Outline the following methods used in testing the quality of milk
i)
Phosphatase
(3
marks)
ii)
Methylene
blue (4
marks)

SECTION B
Answer only TWO questions
(20 marks each)
4. a) Explain
the
applications of heat in the control of food poisoning
microorganisms.
(10
marks)

b)
Explain the precautions taken before collecting chlorinated tap water for
bacteria
analysis.
(5
marks)
c)
Discuss aflatoxicosis disease under the following headings:-
i)
Causative
agent.
(2
marks)
ii)
Disease
condition.
(3
marks)

5.
a)
The current floods have caused spread of cholera in many parts of Kenya.
i)
State the causative agent of the above named disease.

(2 marks)

ii)
Explain how the agent in transmitted

(3 marks)

iii)
Name one isolating media for the agent

(1 mark)

iv)
Outline Oxidase test for the agent causing cholera (4 marks)

b)
Outline the presumptive test as used in enumeration of bacteria in water
samples.

(10 marks)
6.
a)
Name a selective media and a diagnostic test used for isolation and
identification of the following pathogenic bacteria found in food
i)
Clostridium perfrigens (2
marks)
ii)
Staphylococcus aureus
(2
marks)
iii)
Campylobacter jejuni

(2 marks)
iv)
Bacillus cereus

(2 marks)
b)
Explain the following processes in sewage treatment
i)
Primary

(4 marks)
ii)
Tertiary (8
marks)
Page 2 of 2

Food And Water Microbiology Question Paper

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