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Essential Of Molecular Biology Question Paper

Essential Of Molecular Biology

Course:Bachelor Of Education Science

Institution: Kenyatta University question papers

Exam Year:2009

KENYATTA UNIVERSITY
UNIVERSITY EXAMINATIONS 2009/2010
FIRST SEMESTER EXAMINATION FOR THE DEGREE OF BACHELOR OF
SCIENCE AND BACHELOR OF EDUCATION

SZL 303: ESSENTIAL OF MOLECULAR BIOLOGY

DATE: Tuesday
22nd December 2009
TIME: 2.00pm – 4.00pm

INSTRUCTIONS

Answer All questions in Section A and One question in Section B. Illustrate your
answers with diagrams and give examples wherever appropriate.
SECTION A
Answer All questions (40 marks)
1.
Explain the concept of variable number tandem repeats.
2.
Explain the salient characteristics of a probe and give at least two examples.
3.
Describe briefly the following terminologies:
a)
Genomics
b)
Bioinformatics
4.
Distinguish recombinant from genomic DNA.
5.
Outline reverse transcription in a retrovirus.
6.
Briefly explain the causes of nonsense mutations and their effects on gene expression.
7.
Describe the shotgun technique for obtaining suitable cloning vectors.
8.
Explain insulin synthesis using rDNA technology.

SECTION B
Answer any One question (30 marks)
9.
Discuss transcriptional regulation in prokaryotic cells.
10.
Discuss an ju vivo and in vitro system of gene implication and their applications.