One requires a microscope, transparent ruler marked in millimeters and a prepared slide of cells.
With the low power objective lens in place, keep a transparent ruler on the stage of the microscope.
Focus so that the millimeters marks on the ruler are seen as thick dark lines.
Estimate the diameter of the field of view by counting the one millimeter spaces between the first mark and the last one across the field of view.
Count only the spaces between two thick dark lines.
Convert the diameter of the field of view from millimeters to micrometres.
Remove the ruler and place the prepared slide of cells.
Count the number of cells along the diameter of the field of view.
Calculate the diameter of one cell using the formula:
Cell diameter = diameter of the field of view in micrometers divided by number of cells.
vindori answered the question on April 17, 2018 at 13:32
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