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Define aseptic techniques

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· Aseptic technique/“sterile technique,” is a set of routine measures that are taken to prevent cultures, sterile media stocks, and other solutions from being contaminated by unwanted microorganisms (i.e., sepsis).
· Since the goal of a biologist is to grow microorganisms or eukaryotic cells without the introduction of extraneous organisms, aseptic techniques are crucial for accurate and meaningful experimentation. · One should always remember that a completely sterile working environment does not exist. · However, there are a number of simple, common sense procedures that will reduce the risk of culture contaminations.
· The aseptic techniques control the opportunities for contamination of cultures by microorganisms from the environment, or contamination of the environment by the microorganisms being handled.
Examples of aseptic technique are:
· Cleaning and disinfecting lab surfaces prior to use
· limiting the duration that cultures or media are uncapped and exposed to the air
· keeping petri dishes closed whenever possible
· effectively sterilizing inoculating loops and other equipment that comes into contact with cultures or media, and
· Avoiding breathing on cultures or sterile instruments.

Titany answered the question on September 10, 2021 at 12:21

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