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Describe an experiment you would carry out in order to demonstrate that phloem transports manufactured food substances in a plant

Describe an experiment you would carry out in order to demonstrate that phloem transports manufactured food substances in a plant
a. Ringing Experiment
b. Use the radio-tracers
c. Collecting exudate from stylets of aphids

Answers

Isacko
a. Ringing experiment

cut a ring in the bark including the phloem from the stem of a woody plant

phloem is found next to or just beneath the bark

observe daily for some time(more than three weeks)

a swelling of the bark appears above the ring

this is due to accumulation of food from leaves

the bark of a second similar plant is removed carefully leaving the phloem intact

a swelling does not appear

ii) Use the radio-active tracers

plant is exposed to carbon containing radio-active carbon C14

C14 is found in the end products of photosynthesis

It is finally detected in phloem

C14 is found to move in both directions

iii) Collecting exudate from stylets of aphids

aphids feed on certain plant phloem using their stylets

aphid mouth parts are dissected using a sharp razor

exudes from the mouth parts are collected and then analyzed

sucrose is found to be a major component of the exudates

this proves that phloem translocates manufactured food substances
Mohaissack answered the question on October 4, 2017 at 14:06

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