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04-A:Histology Question Paper

04-A:Histology

Course:

Institution: Kenya Medical Training College question papers

Exam Year:2009

KENYA MEDICAL TRAINING COLLEGE KITUI

DEPARTMENT OF MEDICAL LABORATORY SCIENCES

P.O BOX 711

KITUI

SEPTEMBER 2009 MLS CLASS END OF 2ND YEAR EXAM

HISTOLOGY PAPER

DATE 16/12/2010

TIME 2 HOURS

8:30AM-10:30PM

INSTRUCTIONS TO THE CANDIDATE
1.Write your college number on all sheets of paper used.
2.Do not write your name on the sheets of paper used.
3.Attempt all questions in section I and II.
4.Cycle the correct response(s) in section I.
5.For any wrong response you will be deducted half a mark.
6.The paper takes 2 hours.

SECTION I (M.C.Q 40 MARKS)

1.Which one of the following is a cytoplasmic inclusion?
a)fat
b)lysosome
c)mitochodria
d)centriole
e)mucin

2.The role of endoplasmic reticulum includes:
a)digestive
b)delivery
c)cell division
d)nutritional
e)protection

3.Another term used to describe mitosis is:
a)karyohexisis
b)karyolysin
c)karyokinesis
d)meiosis
e)pyknosis

4.Telophase is ..............stage of cell division
a)first
b)second
c)third
d)fourth
e)fifth

5.Characteristics of an inflamed cell include:
a)calor
b)valor
c)cryostatic
d)artefact
e)black

6.Which one of the following is a surface softener?
a)perenyl fluid
b)formaldehyde
c)orth''s fluid
d)muller''s fluid
e)clarkes''s fluid

7.Stratified squamous epithelial cells are found in:
a)cornea of the eye
b)nails
c)lining of the mouth
d)lung
e)liver

8.Pituitary glands are found at:
a)the grey part of the brain
b)white part of the brain
c)interior part of the brain
d)spine
e)base of the brain

9.What are the signs of a cell undergoing autolysis?
a)swelling
b)fragmentation
c)a homogenous mass
d)condensation
e)empty spaces

10.Characteristics of a good fixative include:
a)must activate enzymes
b)must penetrate evenly and poorly
c)must be anti-bacterial
d)must have a negative effect on staining
e)must preserve tissues in a lite like manner

11.Secondary fixation is done after fixation of a tissue in:
a)mercuric chloride
b)susa
c)zenker fluid
d)normal saline
e)hellys fluid

12.The following are cytoplasmic fixatives except:
a)muller
b)carnoy
c)flemming
d)schaudin
e)orths

13.Which one of the following is good for bone marrow fixation?
a)trichroacetic acid
b)acetone
c)carnoys
d)clarkes fluid
e)zenker fluid

14.Gooding and stewarts solution has the following ingredients:
a)formic acid
b)nitric acid
c)distilled water
d)hydrochloric acid
e)trichroacetic acid

15.Methods of identifying the end point of de-calcification include:
a)x-ray method
b)chelating method
c)electrolyte method
d)chemical method
e)ion exchange resin method

16.Yellow colouration in de-calcification is removed by:
a)1% uric acid
b)1% urea
c)nitious acid
d)nitric acid
e)10% urea

17.All of the following are dehydrating agents except:
a)butanol
b)dioxane
c)acetone
d)benzene
e)toulene

18.Among the following which one has the highest specific gravity?
a)xylene
b)cedar wood oil
c)chloroform
d)carbon tetra chloride
e)toulene

19.Ultrasonic vibration is used during:
a)sectioning
b)staining
c)tissue processing
d)tissue floatation
e)microscopy

20.Which one of the following uses Ben Heiffor type of knife?
a)rotary microtome
b)cambridge microtome
c)cryostat
d)freezing microtome

21.The finest type of hones inlcude:
a)carborudum
b)arkanas
c)belgium yellow
d)nicks
e)belgium black

22.Simple rules adhered to when honing the microtome knife automatically
a)Never use the same glass plate for knives of different lengths
b)Use two surfaces of glass hone plate for coarse honing
c)Use both surfaces of glass plate for fine honing
d)Always centre the knife precisely
e)Use xylene for cleaning the oil before use

23.What is the remedy for scores?
a)increase the knife tilt
b)clean the knife edge
c)reduce the tilt
d)apply ice to cool the knife
e)hone the knife

24.Glycerine is made up of the following:
a)gelatine
b)starch paste
c)thymol
d)serum
e)white egg

25.Aqeous media include:
a)DPS
b)DPX
c)Xylene balsam
d)Farrant media
e)glycerine jelly

SECTION II (60 MARKS)
1.Explain the microtome knife profile with the aid of a diagram (20 marks)

2.a)Describe the characteristics of a cell undergoing autolysis (10 marks)
b)Describe how chelating agents are used as a means of de-calcification and how it is achieved(10 marks)

3.Explain the following terms: (20 marks)
a)stropping
b)causes of section detachment from slide
c)characteristics of good mounting media
d)cytoplasmic inclusions
e)secondary fixation

04-A:Histology Question Paper

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