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04-A:Histology Question Paper

04-A:Histology

Course:

Institution: Kenya Medical Training College question papers

Exam Year:2009

KENYA MEDICAL TRAINING COLLEGE

DEPARTMENT OF MEDICAL LABORATORY SCIENCES

COLLEGE EXAMINATION 2009

BASIC DIPLOMA IN MEDICAL LABORATORY SCIENCES

PAPER:04-A HISTOLOGY (100 MARKS)

INSTRUCTIONS
1. Write you index number on every answer sheet used.
2. Do not write your name on any answer sheet.
3. Attempt both sections I and II

SECTION I COMPRISES OF M.C.Q.
4. Circle the correct answer(s) in every question.
5. Be careful,as half a mark will be deducted for every wrong response.

SECTION II COMPRISES OF ESSAY QUESTIONS
6. Attempt all the questions.Make sure you understand the question before answering.

SECTION I (M.C.Q 40 MARKS)
1.How are floaters avoided when performing papicolon staining technique?
a)By filtering and changing xylene regularly
b)By staining gynaecological smears differently from non gynaecological
c)By avoiding bluing solution
d)By dipping five times or less during dehydration and clearing
e)By lowering alcohol levels to ensure not all the parts of the smear are stained
2.During Orange G 6 stains preparation the following reagents are required
a)Ethyl alcohol
b)Phosphotungstic acid
c)Light green
d)Bismaret brown
e)Lithium carbonate
3.Koilocytes are more prominent in
a)Normal cells
b)Parasite invaded cells
c)Human papiloma virus invaded cells
d)Treponema palidum invaded cells
e)Cancerous cells
4.The role of centrioles in a human cell includes:
a)Digestive and delivery system of the cell
b)Storage of supplies within the cell
c)Protection of the cell
d)Division of the cell during mitosis
e)Carries nutritional value of the cell
5.During metaphase:
a)Nuclear membranes are formed and chromatin dissolves into new nucleus
b)Cytoplasmic membranes become elongated and constrict
c)Nuclear membrane disappears and centrioles arrange themselves at the opposite poles of the cell.
d)Two daughter cells are formed
e)The two chromatids of the chromatin start to repel each other forcing chromosomes to separate
6.Simple squamous epithelial cells are found in:
a)Retina of the eye
b)Gall bladder
c)Tymphanic membrane
d)Loop of henle
e)Salivary glands
7.Which of the following is not of the small intestinal wall?
a)Medulla
b)Serous coat
c)Muscular coat
d)Mucosa coat
e)Sub-mucosa coat
8.Two types of cells located in pituitary gland include:
a)Gama cells
b)Alpha cells
c)Zelta cells
d)Episilon cells
e)Beta cells
9.The ingredients of carnoys fluid include:
a)Absolute ethanol
b)Picric acid
c)Potassium dichromate
d)Chloroform
e)Distilled water
10.Why is mercuric chloride fixative not widely used in histology laboratory?
a)Causes shrinkage and is a slow fixative
b)Causes mercuric chloride pigment
c)Tissues fixed using mercury can only be demonstrated using an electron microscope
d)Its explosive
e)It can only be used to fix bones which will be de-calcified using x-ray technique
11.Modified millioning is modification of:
a)Zenker fluid
b)Flemming fluid
c)10% Neutral buffered formalin
d)Hellys solution
e)Carnoys fluid
12.Why are chelating agents not used routinely as a means of de-calcification?
a)They are very toxic
b)They form yellow pigments
c)They require electricity
d)They are very slow
e)Zeokard 225 is not usually available
13.How is nitious pigment removed from a tissue
a)washing in running tap water
b)By using 5% urea
c)By using scoH tap water
d)By using 100% urea
e)Using ethanoic acid
14.The melting point of hard paraffin wax is:
a)45-50 degrees Celsius
b)36-37 degrees Celsius
c)55-60 degrees Celsius
d)48-56 degrees Celsius
e)46-48 degrees Celsius
15.The following are advantages of paraffin wax embedding medium
a)It is only good for thin sections
b)Reasonable speeding of processing
c)Serial sections are not formed
d)Durable blocks
e)Suitable for ultra microtome machine
16.Cambridge microtome best uses the following types of knives
a)Tool wedge knife
b)Plate glass knife
c)Plano concave knife
d)Plane wedge knife
e)Ben heiffor knife
17.Simple microscopes have two types of convex lenses:
a)Nose piece
b)Eye piece
c)Diaphragm
d)Magnification lens
e)Objective lens
18.Fluorescence microscopes uses the following primary natural substances as florescence
a)Chloroplasts
b)Thioflavine
c)Riboflavine
d)Acridine orange
e)Curamine
19.The prisms used in polarized microscopy include:
a)Exciter fitter
b)Polarizer
c)Barrier filter
d)Araldite
e)Analizer
20.Composition of glycerine jelly include:
a)Egg albumin
b)Hcl
c)Gelatine
d)Thymol
e)Serum
21.Examples of mordants include:
a)Potassium hydroxide
b)Iron
c)Chromium
d)Methyl blue
e)Phenol
22.Physical theory of staining includes all of the following except:
a)Permeability
b)Density
c)Solubility
d)Adsorption
e)Coloured compound
23.Natural pigments include:
a)Asbestos fibre
b)Silica
c)Pink pigments
d)Chrome pigments
e)Mercury pigments
24.In turnball reactions non-ferrous reaction pigments stains:
a)Blue
b)Red
c)Unstained
d)Black
e)Yellow
25.Ripening agents in phospotungestic haematoxylin include:
a)Mercuric oxide
b)Sodium iodate
c)Mercuric chloride
d)Picric acid
e)Eosin

SECTION II
1.With the aid of a well labelled diagram discuss vacuum impregnation technique (20 marks)
2.Briefly explain vital staining techniques (10 marks)
3.Write short notes on Zenker fluid (10 marks)
4.Describe the following terms (20 marks)
a)Ion exchange resins
b)Stropping
c)Accentuators
d)Possible causes of section detachment from slide.

04-A:Histology Question Paper

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