Metabolic Regulation And Integrated Metabolism Question Paper

Metabolic Regulation And Integrated Metabolism

Course:Bachelor Of Science In Biotechnology

Institution: Kenyatta University question papers

Exam Year:2009

KENYATTA UNIVERSITY
UNIVERSITY EXAMINATIONS 2009/2010
FIRST SEMESTER EXAMINATION FOR THE DEGREE OF
BACHELOR OF SCIENCE
(BIOCHEMISTRY AND BIOTECHNOLOGY)

SBC 330: METABOLIC REGULATION AND INTEGRATED METABOLISM

DATE:
Monday 21st December 2009
TIME:
8.00am – 10.00am

INSTRUCTIONS
SECTION A
Answer any two Questions (20 marks each)
1.
a)
Outline the post-transcriptional processing of m-RNA in Eukaryotes.

[10marks]

b)
With examples for each, explain the following terms:
i)
cis-and
trans-elements
[4marks]

ii)
promoters

[3marks]
iii)
DNA
Looping
[3marks]

2.
a)
i)
Write the reactions for Hexokinase and Glucokinase enzymes.

[2marks]
ii)
State
their
tissue
specificity
[2marks]

b)
Using kinetic parameters of the above enzymes (a) and relevant glucose
transporters
explain
hepatic
regulation
of:
i)
tissue
glucose
level
[6marks]
ii)
substrate
availability
and
rate of glycolyis.
[6marks]

Page 1 of 2

c)
State the role of insulin in the regulation of Hexokinase/Glucokinase

enzymes and glucose transporters function in the skeletal muscles.

[4marks]
3.
a)
State the tissue specificity of the following:

i)
SREBP-2 (sterol responsive element binding protein 2)

ii)
SREB-1c (sterol responsive element binding protein 1c) [3marks]

b)
Outline the regulation of gene expression by

i)
SREBP-2

[6marks]

ii)
SREBP-1c

[4marks]

c)
i)
List the responsive elements involved in the above (b) regulation.

[2marks]
ii)
Explain
the
nutritional regulation of gene expression via SREBP-2
and
SREBP-1c. [5marks]
SECTION B
Answer all Questions (10 marks each)
4.
a)
Using equation Write the reaction of
i)
Pyruvate
Kinase
ii)
Pyruvate
Carbocylase
iii)
Phosphoenolypyruvate
carboxykinase [3marks]

b)
Explain the regulation of the above enzyme (a) as a substrate cycle in the

liver.

[7marks]

5.
a)
Explain how glucagon regulates glycogen breakdown in the liver.

[4marks]

b)
Explain how the following compounds regulate glycogen phosphorylase in

exercising muscles:

[6marks]

i)
AMP

ii)
Ca2+

iii)
adrenalin.

6.
a)
Outline the formation of the inositol triphosphate as a second messenger.

[6marks]

b)
Explain how inosiol triphosphate regulated eiscosanoid metabolism.

[4marks]
Page 2 of 2

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