Gene Cloning Question Paper

Gene Cloning

Course:Bachelor Of Science

Institution: Kenyatta University question papers

Exam Year:2009

KENYATTA UNIVERSITY
UNIVERSITY EXAMINATIONS 2009/2010
FIRST SEMESTER EXAMINATION FOR THE DEGREE OF BACHELOR OF
SCIENCE (BIOCHEMISTRY AND BIOTECHNOLOGY)
SBC 415:
GENE CLONING

==============================================================
DATE: WEDNESDAY 23RD DECEMBER 2009 TIME: 2.00 P.M. – 4.00 P.M.

INSTRUCTIONS

Section A
Answer ANY TWO Questions (20 marks each)

Page 1 of 3

1.
a)
Use gene fragments A and B to answer the questions below
i)
Give a step wise procedure for joining gene fragment A and gene
fragment B using PCR and give the primer sequences. (4 marks)

ii)
Give the annealing temperatures for all the primers to be used.

(3 marks)

ii)
Give the optimum extension time for each PCR reaction.
(3 marks)

b)
Describe how you would mutate the highlighted ‘T’ nucleotide to a ‘G’.
using PCR and give the primer sequences.

(10 marks)

2. Describe two methods used in identification of recombinant clones based the
translation product of the cloned
gene.
(20
marks)

3. a)
Describe the technique of Southern blotting.

(15 marks)
b)
What information can be obtained from a southern blot?

(5 marks)

Section B: Answer ALL Questions (10 marks each)

4. a)
Explain the techniques and the principle used in achieving the following

(4 marks)
i)

Directional
cloning

ii)
Preventing re-circularization of non-recombinant vectors
iii)
Generating a new restriction enzyme site at a blunt end
iv)
Introducing a restriction enzyme site by PCR.

b)
Differentiate between A DNA library and an expression library.

(4 marks)
c)
Differentiate between replacement and insertion vectors. (2 marks)

Page 2 of 3

5. a)
Describe the method of delivering DNA to hosts for
(3 marks)
i)
YAC
vectors

ii)
Lambda phase vectors
iii) Bacterial plasmids
b)
Describe how you would obtain pure mRNA from a crude RNA
preparation.
(7
marks)

6. a)
Outline FOUR qualities of a good cloning vector.
(2 marks)

b)
Describe why plasmids are desirable for use as cloning vectors.

(8 marks)

Page 3 of 3

📘 Access 10,000+ CBC Exams With Marking Schemes

Gene Cloning Question Paper

More Question Papers

Exams With Marking Schemes

End Term 3 Exams

Mid Term Exams

End Term 1 Exams

Opener Exams

Full Set Exams