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Biochemistry Of Microorganisms Question Paper

Biochemistry Of Microorganisms 

Course:Bachelor Of Science

Institution: Kenyatta University question papers

Exam Year:2009



KENYATTA UNIVERSITY
UNIVERSITY EXAMINATIONS 2009/2010
FIRST SEMESTER EXAMINATION FOR THE DEGREE OF
BACHELOR OF SCIENCE
(BIOCHEMISTRY AND BIOTECHNOLOGY)

SBC 334: BIOCHEMISTRY OF MICROORGANISMS

DATE:
Wednesday 30th December 2009
TIME: 8.00am – 10.00am



INSTRUCTIONS:
Section A: Answer Any Two Questions (20 marks each)

1.
a)
Name the bacteria which use lactic acid fermentation.
[3marks]

b)
Write the reactions of heterolactic fermentation by bacteria using


phosphoketolase pathway of glucose breakdown.

[9marks]

c)
State the natural habitats of lactic acid producing bacteria [4marks]

d)
Explain the importance of Lactobacillus in human health. [4marks]


2.
a)
i)
Draw the respiration chain of Paracoccus.
[4marks]


ii)
State the types of respiration for this bacterium.
[1mark]

b)
Write the reactions of hydrogen and electron transfer in the respiration
chain
of
Paracoccus.
[11marks]

c)
Indicate the sites of the formation of ATP in the above respiration


chain (a)






[2marks]
Page 1 of 2

d)
What is the metabolic advantage of interconversion of NADH and
NADPH
in
Paracoccus?
[2marks]

3.
a)
Name the bacteria growing on the methanol/formaldehyde substrates.










[2marks]

b)
Write the reactions of formaldehyde assimilation via serine pathway.










[12marks]

c)
State the fate of produced Acetyl-CoA and NADH in the above cycle (b).










[2marks]

d)
Explain the environmental role of the methanol utilizing bacteria.










[4marks]

Section B: Answer All questions (10 marks each)
4.
a)
Outline the light-harvesting process of photosynthentic unit of Purple


bacteria.






[4marks]

b)
With the aid of diagram, show the electron and hydrogen transfer in


reaction center and respiration chain during light-harvesting and Purple


bacteria.






[6marks]
5
a)
Illustrate the reactions of catechol ortho-cleavage by bacteria.











[7marks]

b)
State the importance of this metabolism in the biological cycles.










[3marks]

6.
a)
Name the non-methanogenic bacteria, which ferment biological polymers


and produce substrates for methanogens in the digestive tract of


ruminants.






[2marks]

b)
List the products of fermentation by non-methanogenic bacteria, which are


used as substrates for methane synthesis by methanogens. [2marks]

c)
Write the reactions of methane production by the methanogens in


ruminants.






[6marks]


Page 2 of 2






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