Molecular Markers Question Paper

Molecular Markers

Course:Bachelor Of Science

Institution: Kenyatta University question papers

Exam Year:2009

KENYATTA UNIVERSITY
UNIVERSITY EXAMINATIONS 2009/2010
FIRST SEMESTER EXAMINATION FOR THE DEGREE OF BACHELOR OF
SCIENCE
(BIOCHEMISTRY AND BIOTECHNOLOGY)

SBC 416:
MOLECULAR MARKERS

==============================================================
DATE: THURSDAY 24TH DECEMBER 2009 TIME: 11.00 A.M. – 1.00 P.M.

INSTRUCTIONS

SECTION A– 20 MARKS:
Answer ANY TWO Questions.

1.
Using specific examples explain the current applications and future potential of
molecular marker technology in ONE of the following fields.
a)
Forensic
investigations
b)
Human
medicine

2.
Explain the following techniques and their relative merits in detecting DNA
polymorphisms
a)
Gel

Electrophoresis.
(8
marks)
b)
Capillary
Array
Electrophoresis
(5
marks)
c)
Matrix-Assisted
laser
Desorption.
(7
marks)

Page 1 of 2

3.
Discuss any five characteristics considered when choosing markers for DNA
fingerprinting.

(20
marks)

SECTION B (10 MARKS EACH)
Answer ALL Questions

4.
Briefly explain marker assisted selection.

(10 marks)

5. Explain:
a)
Why molecular marker systems are better than “classical” markers in
detecting
variation?

(5
marks)

b)
The use of STRs in genome mapping

(5 marks)

6.
Compare the relative advantages and disadvantages of the following molecular
markers.

a)
SNPs

(2 ½ marks)

b)
SSRs

(2 ½ marks)

c)
Allozymes

(2 ½ marks)

d)
RAPDs

(2 ½ marks)

Page 2 of 2

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