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Immunotechniques Question Paper

Immunotechniques 

Course:Bachelor Of Science

Institution: Kenyatta University question papers

Exam Year:2009



KENYATTA UNIVERSITY
UNIVERSITY EXAMINATIONS 2009/2010
FIRST SEMESTER EXAMINATION FOR THE DEGREE OF BACHELOR OF
SCIENCE (BIOCHEMISTRY/BIOTECHNOLOGY)

SBC 318:
IMMUNOTECHNIQUES


DATE: Wednesday, 30th December, 2009
TIME: 11.00 a.m. – 1.00 p.m.
------------------------------------------------------------------------------------------------------------
INSTRUCTIONS:
Answer TWO questions in Section A and ALL the questions in Section B
Section A
(40 marks)
1.
Discuss the principle and applications of the Enzyme linked Immunosorbent
Assay (ELISA).






(20 marks)

2.
Discuss the principle and applications of the western Blot
(20 marks)

3.
Describe the principle and procedure for purification of antibodies by saturated
ammonium sulphate and affinity chromatography
(20
marks)

Section B
(10 marks each)
4.
Describe the various classes of
antibodies
(10
marks)

5.
Explain the principle for production of monoclonal antibodies
(10 marks)

Page 1 of 2


6.
Briefly describe the following
procedures

a)
Double immunodiffusion
b)
Immunoelectrophoresis
c)
Countercurrent electropjhoresis
d)
Assay for complements
(10 marks)


Page 2 of 2




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