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Fermentation Technology Question Paper

Fermentation Technology 

Course:Bachelor Of Science

Institution: Kenyatta University question papers

Exam Year:2009



KENYATTA UNIVERSITY
UNIVERSITY EXAMINATIONS 2009/2010
FIRST SEMESTER EXAMINATION FOR THE DEGREE OF BACHELOR OF
SCIENCE (BIOCHEMISTRY/BIOTECHNOLOGY)
SBC 417: FERMENTATION TECHNOLOGY

DATE: MONDAY 28TH DECEMBER 2009

TIME: 11.00 A.M. - 1.00 P.M.

SECTION A: Answer TWO questions (20 marks each).

1.
Explain media sterilization in relation to

a)
Integration of the 1st
order
reaction.
(7
marks)
b)
Temperature
and
reaction
rate
constant
(6
marks)
c)
Fractional
reduction
in
viable
organism
(7
marks)

2.
a)
Explain the growth pattern of microbes in a batch reactor
(6 marks)

b)
Derive the equation for growth of microbes during


i)
the log phase





(4 marks)
ii)
the
chemostat
stage
(4
marks)

c)
What happens to a continous culture if the substrate is depleted below


the level that supports the growth rate as a result of dilution?
(6 marks)

3.
A pilot sterilization process is carried out in 108dm3 vessel with a medium
containing
103 orgnaisms/cm3 with a probability of 1 in hundred micro-

organisms getting contaminated,

a)
Calculate the overall del factor (?) of the reaction.

(5 marks)

b)
Calculate the overall del factor (?) of the reaction if scaled up by


100 times for an industrial production.



(5 marks)

c)
Draw a well labeled diagram of a batch reactor and list the uses of the
labeled
parts
of
the
reactor.
(5
marks)

d)
What are the differences between a batch culture and continues culture.











(5 marks)
Page 1 of 2




SECTION B: Answer all questions (10 marks each).

4. a) Explain
the
causes
of deviation from an ideal condition in a


fermentation reaction.





(4 marks)

b)
How would you improve the strain of a secondary metabolite
producing
culture.
(6
marks)

5.
a)
How is microbial fermentation processes
classified?
(5
marks)

b)
Briefly describe the function of one of the classes.

(5 marks)

6.
a)
Illustrate the general schematic representation of a fermentation


process.







(6 marks)

b)
What are the advantages of a Fed-batch culture.


(4 marks)








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