Analytical Biochemistry I Question Paper

Analytical Biochemistry I

Course:Bachelor Of Science

Institution: Kenyatta University question papers

Exam Year:2009

KENYATTA UNIVERSITY
UNIVERSITY EXAMINATIONS 2009/2010
FIRST SEMESTER EXAMINATION FOR THE DEGREE OF BACHELOR OF
SCIENCE (BIOCHEMISTRY/BIOTECHNOLOGY)

SBC 332:
ANALYTICAL BIOCHEMISTRY I

DATE: Monday, 28th December, 2009

TIME: 8.00 a.m. – 10.00 a.m.
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INSTRUCTIONS:
Section A:
Answer any TWO questions (20 marks each)
1.
Explain the principle and procedure of liquid scintillation counting

2.
Describe the theory and procedure of isoelectric focusing

3.
Explain the procedure of protein separation by ion – exchange chromatography

Section B:
4.
Explain the principle of a pulse – chase experiment

5.
Describe the principle of a magnetic sector mass spectrometer

6.
In biochemical analysis, many enzymes – catalyzed reactions can be monitored by
conversion of NAD(P) to NAD(P)H. This is done by measuring the absorbance
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at two wavelengths (260 nm) where both reduced and oxidized forms absorb and
at 340 nm where only NAD(P)H absorbs.

Calculate the concentration of NAD(P) and NAD(P)H in a mixed solution from
the following data obtained using 1 cm cuvet given that the molar absorptivity at
260 nm = 1.05 x 10(4) for NAD(P)H and 1.84 x 10(4) for NAD(P). At 340 nm
only NAD(P)H absorbs with molar absorptivity 6.22 L/mol/cm. The mixed
solution gave absorbances of 1.32 and 0.382 at 260 nm respectively.
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