Fundamentals Of Biotechnology Question Paper

Fundamentals Of Biotechnology

Course:Bachelor Of Science

Institution: Kenyatta University question papers

Exam Year:2009

KENYATTA UNIVERSITY
UNIVERSITY EXAMINATIONS 2009/2010
FIRST SEMESTER EXAMINATION FOR THE DEGREE OF BACHELOR OF
SCIENCE (BIOCHEMISTRY / BIOTECHNOLOGY)

SBC 220N: FUNDAMENTALS OF BIOTECHNOLOGY
=================================================================
DATE: WEDNESDAY 30TH DECEMBER 2009
TIME: 8.00 A.M. – 10.00 A.M.

INSTRUCTIONS

Answer ANY Two Questions (Twenty marks each)

1.
Describe the procedure for
a)
Constructing
a
cDNA
library.
(10
marks)

b)
Making a genomic library in baceteriophage lambda vector.
(10 marks)

2.
Describe the application of recombinant DNA technology in agriculture. (20 marks)

3. a) Describe
the
technique and procedures of
i)
Mutation
breeding.
(5
marks)
ii)
Somaclonal
variation
breeding. (5
marks)

b)
List the advantages and disadvantages of Somaclonal / Mutation breeding.

(4 marks)

c)
Give some examples of successes of somaclonal / Mutation breeding.

(6 marks)

Page 1 of 2

SECTION B.
Answer ALL Question (10 marks each)

4.
Outline the chronological events that have led to the development of modern day
recombinant
DNA
technology. (10
marks)

5.
a)
Explain the basic steps in a gene cloning experiment

(8 marks)

b)
Give examples of an enzyme that generates

i)
Blunt ends

(1 mark)

ii)
Sticky ends

(1 mark)

6.
a)
State FOUR qualities of a good cloning vector.

(4 marks)

b)
Explain why bacteriophage lambda is a good cloning vector.
(6 marks)
Page 2 of 2

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