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Analytical Techniques Question Paper

Analytical Techniques 

Course:Bachelor Of Science In Medical Laboratory Science

Institution: Kenyatta University question papers

Exam Year:2009



KENYATTA UNIVERSITY
UNIVERSITY EXAMINATIONS 2009/2010
FIRST SEMESTER EXAMINATION FOR THE DEGREE OF BACHELOR OF
SCIENCE (MEDICAL LABORATORY)

HML 308:
ANALYTICAL TECHNIQUES

DATE: Monday, 28th December, 2009

TIME: 11.00 a.m. – 1.00 p.m.
------------------------------------------------------------------------------------------------------------
INSTRUCTIONS:
SECTION A:
Attempt ALL questions. (10 marks each)
Q.1
Describe the various methods used to interpret the results of protein
electrophoresis

(10
marks)

Q.2` a)
Describe the standard operating procedure (SOP) for oval glucose
tolerance
test
(OGTT)
(5
marks)

b)
Briefly describe the pre – analytical errors in specimen handling and
processing that would compromise the quality of laboratory results.










(5 marks)

Q.3 a) Describe
the
characteristics of a good reagent kit

(3 marks)

b)
Outline the advantages of automation in clinical laboratory and mention
three factors that necessitated its introduction.

(4 marks)

c)
Match the analysis given in column A with respective analytical methods
given in column B.
Page 1 of 2




COLUMN A


COLUMN B


- Blood glucose


- I.F.C.C. standard method
-
ALT
-
Enzyme
linked
fluorescence
assay
-
CA
125
-
Bromo
Cresal
given
(BCG)
-
Albumin
-
Ber
the
lot
-
Uric
acid
-
Hexokinare
-
BUN
-
Uricase/Peroxidose











(3 marks)

SECTION B:
Answer any TWO questions (20 marks each)
Q.4 Discuss:-

a)
Factors that govern the rate of enzyme catalyzed reactions (10 marks)

b)
Factors that affects enzyme levels in plasma/serum.
(10 marks)

Q.5
You are a laboratory manager in a busy clinical laboratory in a public hospital.
Discuss the mechanisms you would put in place to ensure acquisition of a good
analytical
machine.
(20
marks)

Q.6
a)
Differentiate between internal and external quality control programmes.










(6 marks)

b)
The internal quality control (1Qc) results for AST for days 1, 2, 3, 4, 5, 6,
7, 8, 9 and 10 in the month of October 2009 were as follows:- (20, 15, 20,
20, 15, 20, 15, 25, 20 and S) U/L respectively.
The 1Qc range was (15 – 25) U/L.
i)
Determine the 1Qc target value


(2 marks)
ii)
Determine the 1Qc 1SD (one standard deviation)
(2 marks)
iii)
Determine the 1Qc standard deviation for each day and plot this on
a levy Jenning’s quality control chart provided.
(6 marks)


iv)
What could be the possible causes and remedies for 1Qc results for
day
10.
(4
marks)
Page 2 of 2






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