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Microbiology Question Paper
Microbiology
Course:Diploma In Medical Laboratory Science
Institution: Kenya Medical Training College question papers
Exam Year:2009
KENYA MEDICAL TRAINING COLLEGE
DEPARTMENT OF MEDICAL LABORATORY SCIENCE
1ST YEARS(SEPTEMBER 2009 CLASS)-TERM II 2010
MICROBIOLOGY END OF TERM EXAMINATION
Section A; Choose one most correct answer for each question,0.5 marks will be deducted for every wrong answer, avoid guess work(Total 25 marks)
1. Bacteria that grow in 1-10% oxygen are referred to as:
a.) Halophilic
b.) Facultative
c.) Psychrophiles
d.) Microacriphiliac
e.) Anaerobic
2. Acid butt, acid slant with gas but no hydrogen sulphide in triple sugar iron (TSI)are suggestive of
a.) Escherichia coli
b.) Shigella
c.) Salmonella
d.) Pseudomonas
e.) Citrobacter
3. Negative staining is employed in the laboratory in identification of
a.) Staphylococcus aureus
b.) Enterococcus faecalis
c.) Cryptacoccus neofomans
d.) Vibrio cholera
4. Which of the following ingredients makes laboratory culture media selective
a.) Bile salts
b.) Blood
c.) Serum
d.) Glucose
e.) X and V factors
5. Organisms that hydrolyses tryptophan to form indole is/are
a.) Proteus mirabilis
b.) Citrobacter freundii
c.) Entero bacter cloacae
d.) Pseudomonas aeruginosa
e.) Escherichia coli
6. Sterization by dry heat is achieved at
a.) 100c for one hour
b.) 60c for 30 minutes
c.) 160c for 30 minutes
d.) 160c for 1-2 hours
7.) Specimen not containing commensals includes
a.) Sputum
b.) Urogenital specimen
c.) Pus
d.) Eye discharges
8. Bacteria that grows at 25-40c arte termed as
a.) Psychrophiles
b.) Mesophiles
c.) Halophiles
d.) Commensals
9. Cryptococcus neofomans is an opportunistic organism isolated from
a.) Blood
b.) Urine
c.) Cerebrospinal fluid
d.) Stool
10. The following ingredients do not make culture media selective
a.) Antibiotics
b.) Bile salts
c.) Blood
d.) Dyes
11. The fundamental unit of a living body is
a.) Cell
b.) Cell wall
c.) Cytoplasm
d.) Nucleus
e.) Cell membrane
12. The following are examples of IMViC reactions for identification of familly eneterobacteriacae except
a.) Indole test
b.) Citrate utilization
c.) Methylred test
d.) Voges proskaeur test
e.) Coagulase
13. Virulence of micro organisms is not determined by
a.) Ability to adhere and colonize
b.) Host temperature and pH
c.) Invasiveness
d.) Ability to evade hast defence machanisms
14. Bunsen burner is mainly used for sterilization of
a.) Glassware
b.) Culture media
c.) Wireloop
d.) Surgical equipments
15 Heat fixing of bacteria smear before staining is not aimed at
a.) Killing bacteria
b.) Firmly affixing the smear on the slide
c.) Allowing sample to more readily take up the stain
d.) Maintaining the morphological features of the micro organism
16. Simple staining procedures includes
a.) Ziehl nelson staining
b.) Gram staining
c.) Auramine staining
d.) Methlene blue staining
17. The following does not affect staining
a.) Temperature and time
b.) Nature of the organism
c.) Mordants and accelerators
d.) P.H of the staining solution
18. Biological method to test completeness of sterilization employs
a.) Bacillus anthraxis
b.) Bacillus subtilis
c.) Bacillus stereothermophillus
d.) Bacillus enteritidis
19. Heating during ziehl nelson staining enhamces
a.) Entry of the primary dye through the cell wall of acwid fast bacteria
b.) Washing out of primary dye from the cell wall of acid fast bacteria
c.) Affixing the bacteria onto the bacteria
d.) Presevation of morphology of the bacteria
20. Which of the followijng is not symbioitic association
a.) Opportunism
b.) Commensalism
c.) Parasitism
d.) Mutualism
21. The following are sugars found in triple sugar iron agar except:
a.) Glucose
b.) Sucrose
c.) Lactose
d.) Maltose
22.) Virulence is a term means
a.) Degree of pathogenity
b.) Ability of microorganism to cause diseaese
c.) Ability of an organism to debilitate an host
d.) Ability to adhere to an host
23. Which of the following is a zoonotic disease
a.) Anthrax
b.) Salmonellosis
c.) Bacillary dysentery
d.) Botulism
24. Classification of bacteria into different risk groups takes into account the following except
a.) Pathogenicity of the microorganism
b.) Mode of transmission and host range of the organism
c.)Local availability of effective treatment
d.) Social economic impact of the disease
25. The "germ theory of disease" wasa propased by_______in ______
a.) Lois Pasteur/1857
b.) Theodor schewan/1836
c.) Robert Koch/1876
d.) Joseph lester/1867
SECTION B; SHORT ANSWER AND ESSAY QUESTIONS (35 MARKS)
ANSWER ALL QUESTIONS
1.) State four Koch's postulates about disease agents in relation to diseases that led to development of current culture techniques as employed in isolation of bacteria.(4 marks)
2.) State the principles of the following staining procedures citing examples of microorganisms stained with the stains and their staining reactions (4 marks)
a.) Gram staining
b.) Ziehl Neelson staining
3.) State at least four basic elements required for disease transmission(4 marks)
4.) With specific examples, state the principles of the followinjg biochemical reactions(4 marks)
a.)Coagulase test
b.) Urease test
5.) Describe the following symbiotic relationships (6 marks)
a.) Commensalism
b.) Mutualism
c.) Parasitism
6.) Outline five W.H.O recommended code of p-ractice for medical laboratory personnel (5 marks)
7.) Describe classification of microorganism in terms of risk to the laboratory personnel and the community citing relevant examples (4 marks)
8.) State four universal safety precautions applied in microbiology laboratory (4 marks)
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