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Kst 513: Agricultural Biotechnology Question Paper

Kst 513: Agricultural Biotechnology

Course:Master Of Science In Agronomy

Institution: Kenyatta University question papers

Exam Year:2012

KENYATTA UNIVERSITY
UNIVERSITY EXAMINATIONS 2011/2012
INSTITUTION BASED PROGRAMME (DECEMBER SESSION)
EXAMINATION FOR THE DEGREE OF MASTER OF SCIENCE IN AGRONOMY

KST 513: AGRICULTURAL BIOTECHNOLOGY
DATE: THURSDAY, 3RD MAY 2012 TIME: 2.00 P.M. – 5.00 P.M.

INSTRUCTIONS: Answer any four questions.

1. Write short notes on: (5 marks each)

i) Applications of embryo culture

ii) Mutation induction

iii) Purines and pyrimidines

iv) Processing of mRNA

v) Dominant and codominant DNA markers

2. Murashige and Skoog (MS) stock solutions have been prepared as indicated below:

(i) Macronutrients (x 10)

(ii) Micronutrients (x100)

(iii) Amino acids (x 1000)

(iv) Vitamins (x 1000)

(a) Indicate how much solution (mls) should be drawn from the stock solution to
make five litres of MS media. (8 marks)

(b) Two percent sucrose and 0.8% agar is added to the media above.
Calculate the weights of sucrose and agar added in five-litre media. (10 marks)

(c) Six (6) parts per million BA is added to the media above. Calculate weight of BA added to the media in grams. (7 marks)
Suppose stock solutions have been prepared as follows (25 marks):

(a) BA is 25 mg per 100ml

(b) NAA is 20 mg per 200ml

Calculate volume of stock solutions to make five litres medium with 4 mg per litre BA and 0.2 mg per litre NAA.

4. Discuss application of ten biotechnology tools in crop improvement. (25 marks)

5. A number of issues on GMOs have been resolved. Discuss at least five. (25 marks)

Kst 513: Agricultural Biotechnology Question Paper

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