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Szh 401: Animal Biotechnology Question Paper

Szh 401: Animal Biotechnology

Course:Bachelor Of Science In Animal Health

Institution: Kenyatta University question papers

Exam Year:2015

KENYATTA UNIVERSITY
UNIVERSITY EXAMINATIONS 2014/2015
FIRST SEMESTER EXAMINATION FOR THE DEGREE OF BACHELOR OF SCIENCE
SZH 401: ANIMAL BIOTECHNOLOGY

DATE: MONDAY 24TH NOVEMBER 2014 TIME: 8.00 AM - 10.00 AM

INSTRUCTIONS
answer all questions from section one and any one question from section B. within each section, questions carry equal marks. illustrate your answers with diagrams and give examples
SECTION A: answer all questions (40 marks)
1. describe two methods commonly used in the analysis of DNA
2. briefly explain the principles behind DNA fingerprinting
3. define the restriction endonucleases. using examples describe how restriction endonucleases work
4. describe two methods used in screening of positive clone(s)
5. describe the three methods used in the transfer of genetic material into the host (transformation) in DNA technology
6. why would one modify the gene, why not modify the protein?
7. what are degenerate primers? discuss how they work.
8. briefly explain the DNA shiffling

Section B: Answer only one question (30 marks)
9. i) briefly explain criteria used in the choice of vector used in cloning
ii) briefly explain why DNA is cloned.
10. discuss the application of biothnology in agricultural industry

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