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Bioc 204: Introduction To Biochemistry Question Paper

Bioc 204: Introduction To Biochemistry

Course:Bachelor Of Science In Agriculture, Environmental Science & Agricultural Education & Extension

Institution: Chuka University question papers

Exam Year:2013

CHUKA

UNIVERSITY

UNIVERSITY EXAMINATIONS

SECOND YEAR EXAMINATION FOR THE AWARD OF DEGREE OF
BACHELOR OF SCIENCE IN AGRICULTURE, ENVIRONMENTAL SCIENCE & AGRICULTURAL EDUCATION & EXTENSION

BIOC 204: INTRODUCTION TO BIOCHEMISTRY

STREAMS: B.Sc(AGRIC);BSc(ENSC) TIME: 2 HOURS
B.Sc (AGED) Y2S2

DAY/DATE: WEDNESDAY 7/8/2013 2.30 P.M. – 4.30 P.M.
INSTRUCTIONS:

ANSWER QUESTIONONE AND NAY OTHER TWO QUESTIONS.

QUESTION 1 (30 MARKS)

(a) What are the functions of the following structures in eukaryotic cells?

[5 marks]

Nucleus

Plasma membrane

Endoplasmic reticulum

Ribosomes

Cytoskeleton

(b) Classify the following amino acids according to whether they are neutral, polar, neutral non-polar, acidic or basic. [4 marks]

(c) Convert the following Fischer structures into cyclic Haworth structures. [4 marks]

Draw the ?- andß-anomers of compound (a) above. [1 mark]
(d) What is the pH of a solution prepared by mixing 150 mL of 0.1 M HCl with 300mL of 0.1M sodium acetate (NaOAC) and diluting the mixture of 1L? PKa of acetic acid is 4.76. [4 marks]

(e) Describe the types of non-covalent interactions that promote the stability and functional properties of proteins. [4 marks]

(f) Briefly describe the structure of DNA. [4 marks]

QUESTION 2 (20 MARKS)

(a) Briefly discuss the sources, structures, and functions of the following lipids:
[10 marks]
Triacylglycerols

Wax esters

Phospholipids

(b) (i) Describe the four important properties of enzymes. [4 marks]

(ii) Consider the following reaction:

Give the rate expression for the reaction. [1 mark]

Given the following rate data, determine the order in each reactant and the overall order of the reaction. [5 marks]

[Glycylglycine] (MoL/L) [H2O](MoL/L) Rate (mMoL/S]
0.1 0.1 1 x 10-2
0.2 0.1 2 x 10-2
0.1 0.2 2 x 10-2
0.2 0.2 4 x 10-2

QUESTION 3 (20 MARKS)

(a) (i) What roles do the three most common forms of RNA play in cell
formation? [6 marks]

(ii) Give a major function of each of the following classes of lipids.
[4 marks]
Phospholipids

Sphingolipids

Steroids

Waxes

(b) Describe the four levels of structural organization of proteins. [10 marks]

QUESTION 4 (20 MARKS)

(a) (i) Differentiate the terms in each pair below: [6 marks]

Globular and fibrous proteins

Simple and conjugated proteins

Apoprotein and holoprotein

(ii) To which lipid class does each of the following molecules belong?
[4 marks]

(b) (i) Describe five methods of denaturing proteins. [5 marks]

(ii) Explain five functions of proteins in the body. [5 marks]

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Bioc 204: Introduction To Biochemistry Question Paper

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