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Pmt 436: Methods In Molecular Biology Question Paper

Pmt 436: Methods In Molecular Biology

Course:Bachelor Of Science In Medical Biotechnology

Institution: Maseno University question papers

Exam Year:2016

MASENO UNIVERSITY

UNIVERSITY EXAMINATIONS 2016/2017

FOURTH YEAR SEMESTER EXAMINATION FOR THE DEGREE
OF BACHELOR OF BIOMEDICAL SCIENCES AND TECHNOLOGY WITH
INFORMATION TECHNOLOGY

MAIN CAMPUS

PMT 436: METHODS IN MOLECULAR BIOLOGY

Date: 29th November, 2016 Time: 12.00-3.00pm

INSTRUCTIONS:
.Answer ALL Questions in SECTION A and any other TWO from SECTION B

MASENO UNIVERSITY ISO 9001:2008 CERTIFIED

SECTION A(40 MKS)
1.Outline the basic differences between the following PCR steps;

a.Initialization (2 mks)

b.Annealing (2 mks)

c.Extension (2 mks)

2.Give a brief outline of the following techniques;
a.Nested PCR (3 mks)
b.Quantitative PCR(3 mks)

3.Mention the difference methods in which DNA sequencing can be performed(5 mks)

4.Outline the basic steps involved in routine RNA extraction(4 mks)

5.Write short notes on forensic anthropology(4 mks)

6. Mention the uses of a cDNA library(4 mks)

7.Briefly describe the steps involved in performing Illumina sequencing(4 mks)

8.Outline the basic principle of primer design(3 mks)

9.Describe the strategies of the human genome project(5 mks)

SECTION B
10.Describe the application of DNA sequencing procedures(20 mks)

11.Giving specific examples, discuss s fluorescent detection method(20 mks)

12.
a.Outline the principle of DNA fingerprinting(5 mks)

b.Discuss the steps involved in performing a DNA fingerprinting procedures(15 mks)

13.With the help of diagrams, describe a typical gene cloning experiment(20 mks)

Pmt 436: Methods In Molecular Biology Question Paper

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