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Bioc 0113: Basic Biochemistry Question Paper

Bioc 0113: Basic Biochemistry

Course:

Institution: Chuka University question papers

Exam Year:2013

CHUKA

UNIVERSITY

UNIVERSITY EXAMINATIONS

FIRST YEAR EXAMINATION FOR THE AWARD OF
DIPLOMA IN ANIMAL HEALTH AND PRODUCTION

BIOC 0113: BASIC BIOCHEMISTRY

STREAMS: DIP (ANHE) TIME: 2 HOURS

DAY/DATE: WEDNESDAY 14/8/2013 11.30 A.M. – 1.30 P.M.
INSTRUCTIONS:

ANSWER QUESTION ONE AND ANY OTHER TWO QUESTIONS.

QUESTION 1 (30 MARKS)

(a) Differentiate between prokaryotic and eukaryotic cells. [2 marks]

(b) Describe the functions of the following: [5 marks]

Golgi apparatus
Lysosomes
Mitochondria
Endoplasmic reticulum
Ribosomes

(c) Briefly define the following terms: [3 marks]

pH
Buffer
Weak acid

(d) (i) Calculate the pH of a 3.0 x 10-2M KOH solution . [3 marks]

(ii) Calculate the pH of a mixture of 0.25M acetic acid and 0.1M sodium acetate. The PKa of acetic acid is 4.76. [3 marks]

(e) Give three properties of water that makes it a good medium for biological systems. [3 marks]

(f) Classify the following amino acids as neutral nonpolar, neutral polar, acidic, or basic.

[4 marks
(g) Distinguish between proteins, peptides, and polypeptides. [3 marks]

(h) Convert the following Fischer structure of glucose into cyclic Haworth structures of the two anomers of glucose. Name the resultant anomers of glucose. [4 marks]

QUESTION 2 (20 MARKS)

(a) Describe the structures of the following: [10 marks]

Cellulose
Starch
Sucrose
Lactose
Glycogen

(b) Glycolysis occurs in two stages. Explain the reactions that occur in each stage.
[10 marks]

QUESTION 3 (20 MARKS)

(a) Describe the citric acid cycle. [10 marks]

(b) Discuss the ß-ocidation pathway for degradation of fatty acids. [10 marks]

QUESTION 4 (20 MARKS)

(i) What are the four important properties of enzymes? [4 marks]

(ii) What roles do the three most common forms of RNA play in cell functions?
[6 marks]

(i) Briefly discuss the following classes of carbohydrates giving examples of each
class. [6 marks]

Monosaccharides; Disaccharides; Polysaccharides

(ii) Describe two important roles of the citric cycle. [4 marks]

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Bioc 0113: Basic Biochemistry Question Paper

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